E.coli Susceptibility to Chemical and Physical Agents

Points described here are useful in designing a biosecurity program against E.coli

E.coli does not possess any unique resistance capability
Susceptible to physical and chemical agents like other vegetative gram-negative bacteria
It can be killed at 60 degree Celsius for 30 minutes or 70-degree Celsius for 2 minutes
Thermal fogging can inactivate it (90% reduction in the litter)
It does not affect by cold or freeze
Do not divide at pH below 4.5 or more than 9 BUT NOT KILLED
Some virulent strains (e.g., O157:H7) are acid tolerant, which permits them to pass through the stomach without being killed.
Organic acids are more effective than inorganic acids at inhibiting growth. Treatment with citric, tartaric, or salicylic acids significantly reduce coliform counts in poultry litter
Stabilized chlorine dioxide is highly effective when used as a water disinfectant
UV disinfection of water is a low-cost method of treating drinking water